Thermo Fisher Scientific Human IL-2 Recombinant Protein
인간 IL-2 재조합 단백질로 T 세포 활성화 및 증식 연구에 적합. E. coli 발현 시스템에서 고순도(>95%)로 생산되었으며, 낮은 엔도톡신 수준을 보장. 기능 분석, ELISA, Western blot 등 다양한 생화학적 실험에 활용 가능. 연구용으로만 사용.
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Thermo Fisher Scientific Human IL-2 Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Human |
| Expression System | E. coli |
| Molecular Weight | 15.5 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <0.1 ng/µg |
| Activity | ED50 < 0.5 ng/mL; determined by dose-dependent proliferation of mouse CTLL-2 cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
Carrier-Free
Reconstitution:
Centrifuge the vial briefly before opening to bring contents to the bottom.
Reconstitute the lyophilized protein in 100 mM acetic acid to 0.1–1.0 mg/mL to regain full activity.
Make further dilutions in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.Storage:
Store lyophilized protein at 2–8°C, preferably desiccated.
Upon reconstitution, apportion into working aliquots and store at ≤ -20°C (not in frost-free freezer).
Avoid repeated freeze-thaw cycles.
Target Information
Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages.
IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is shared by IL-4 and IL-7.
The expression of the IL-2 gene in mature thymocytes is monoallelic, representing a unique regulatory mode for precise gene expression control.
Disruption of IL-2-like genes in mice leads to an ulcerative colitis-like disease, indicating its essential role in immune response to antigenic stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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