Thermo Fisher Scientific Phospho-RSK1 (Thr359, Ser363) Recombinant Rabbit Monoclonal Antibody (SU03-65)
RSK1 단백질의 Thr359 및 Ser363 인산화 상태를 검출하는 재조합 토끼 단일클론 항체. Western blot, IHC, ICC/IF에 최적화. 높은 특이성과 일관된 로트 간 품질 제공. 연구용으로 세포 성장 및 MAPK 신호전달 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU03-65 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr359 and Ser363 of Human RSK1 p90 (aa 341–390) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809420 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select the best candidates for production. Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
RPS6KA1 (RSK1) is a growth-factor regulated serine/threonine kinase involved in the MAPK cascade. It controls cellular proliferation and differentiation through phosphorylation of transcription factors. RPS6KA1 contains two nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the MAPK signaling pathway. Its activity is implicated in cell growth and differentiation. Alternate transcriptional splice variants encoding different isoforms have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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