Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), NovaFluor Red 700, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet Series
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue / Yellow Series
• PE, PE-Cyanine Series
• PerCP-Cyanine5.5
• Super Bright Series
• Custom conjugation available

Product Specific Information

Description:
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except for plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II, enhancing BCR signaling and promoting phosphorylation, calcium flux, and proliferation of B cells.

Applications Reported:
Validated for flow cytometric analysis.

Applications Tested:
Tested on mouse splenocytes at ≤0.4 µg/test (100 µL final volume). Optimal cell number: 10⁵–10⁸ cells/test. Titrate for best performance.

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI). Use Invitrogen™ LIVE/DEAD™ Fixable Dead Cell stains instead.

Staining Recommendation:
Use CellBlox™ Blocking Buffer with NovaFluor conjugates.

• Add 5 µL per sample (regardless of number of NovaFluors).
• For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Excitation / Emission / Laser:

• Excitation: 639 nm
• Emission: 700 nm
• Laser: 633–640 nm (Red)

Technology:
NovaFluor conjugates utilize Phiton™ technology, based on nucleic acid dye structures engineered for optimized fluorescent signatures with minimal spillover and spread.

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on all peripheral B cells and certain myeloid leukemia cells. CD19 acts as a co-receptor with the BCR, lowering the activation threshold and amplifying signaling. It is a key regulator in B cell proliferation and differentiation. Mutations lead to hypogammaglobulinemia, while overexpression causes B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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