Thermo Fisher Scientific PCNA Monoclonal Antibody (PC10), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Insect, Mouse, Non-human primate, Rat, Yeast

Published species

Human, Mouse, Sheep

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

PC10

Immunogen

Rat recombinant PCNA polypeptides.

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• Alexa Fluor Plus 647
• Alexa Fluor Plus 750
• FITC
• Request custom conjugation

Form

Liquid

Concentration

0.2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4, with 50% glycerol, 1% BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533017

Target Information

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.