Thermo Fisher Scientific GNAQ Recombinant Rabbit Monoclonal Antibody (JE65-59)
GNAQ 단백질을 인식하는 토끼 단클론 항체로, Western blot과 IHC(파라핀 절편)에 모두 사용 가능. 인간, 생쥐, 랫트 시료에 반응. HEK293 세포 발현, Protein A 정제, 액상 형태. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JE65-59 |
| Immunogen | Recombinant protein within Human GNAQ aa 51–150/359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 0.05% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094237 |
Product Specific Information
- Positive control: Mouse colon tissue, human breast tissue, human thyroid cancer tissue, SH-SY5Y cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate
- Predicted band size: 42 kDa
- Subcellular Location: Cell membrane, Golgi apparatus, Nucleus, Nucleus membrane
Target Information
Guanine nucleotide-binding proteins (G proteins) are heterotrimeric proteins that couple cell surface 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit, resulting in conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits regulate various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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