Thermo Fisher Scientific P-Selectin Monoclonal Antibody (AK-6)
P-Selectin(CD62P)을 인식하는 Mouse Monoclonal Antibody(AK-6)로, Human 및 Rhesus monkey 시료에 반응합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능하며, Protein A 정제 및 PBS buffer에 0.09% sodium azide 포함. 연구용으로 적합.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific P-Selectin Monoclonal Antibody (AK-6)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000–1:5,000 | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | 1:10–1:100 | 1 publication |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AK-6 |
| Immunogen | Human platelet membrane glycoproteins (Target: P-Selectin (CD62P), UniProt ID: P16109-1, Antigen range: 1–830) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538071 |
Available Conjugated Formats
Product Specific Information
- For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.
- Suggested positive control for IHC: tonsil tissue.
- Antigen retrieval not required for paraffin sections, but staining may improve with sodium citrate buffer (pH 6.0).
- Mouse anti-Human CD62P antibody, clone AK-6 recognizes CD62P (P-selectin), also known as Granule membrane protein 140 (GMP140), Leukocyte-endothelial cell adhesion molecule 3 (LECAM3), or Platelet activation-dependent granule-external membrane protein.
Target Information
P-selectin (CD62P, GMP-140, LECAM3) is a 140 kDa type I transmembrane glycoprotein involved in cell adhesion and rolling. It is stored in Weibel-Palade bodies of endothelial cells and α-granules of platelets and rapidly translocates to the cell surface upon activation by thrombin, histamine, or other stimuli.
P-selectin mediates leukocyte interactions with activated endothelial cells or platelets and plays key roles in inflammation, thrombosis, and tissue injury. It binds to sialylated Lewis carbohydrate antigens on neutrophils and monocytes. Clinically, P-selectin aids in differentiating heparin-induced thrombocytopenia with or without thrombosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.