Thermo Fisher Scientific AKT1 Monoclonal Antibody (14E5.A2.B2.H9), PE
AKT1 단백질을 인식하는 Thermo Fisher의 단클론 항체로, PE 형광 표지되어 세포 분석 및 단백질 발현 연구에 적합합니다. Western blot, IHC, Flow cytometry 등 다양한 응용 가능하며, 인간, 마우스, 랫트에 반응합니다.
- 카탈로그번호
- 200-308-I51
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 14E5.A2.B2.H9 |
| Immunogen | Synthetic peptide corresponding to internal residues of human AKT1 protein; produced in mice and developed as a monoclonal antibody. |
| Conjugate | PE (Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate (pH 7.2), 10 mg/mL BSA, 0.5M NaCl |
| Contains | No preservative |
| Storage Conditions | 4°C (short term), -20°C (long term); avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
Additional Formats Available
- Unconjugated
- APC
- Biotin
- FITC
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C prior to reconstitution.
- Reconstitute with deionized water or equivalent.
- Stable at 4°C as undiluted liquid; dilute only before immediate use.
- Centrifuge if solution is not clear after standing at room temperature.
- Do not freeze after reconstitution.
- Store reagent in the dark and handle under subdued lighting.
Antibody characteristics:
Anti-AKT1 antibody recognizes human AKT1 and detects both phosphorylated and unphosphorylated forms.
Cross-reactivity with other species has not been fully tested, but the immunogen shares 85% sequence identity with mouse and 92% with rat, suggesting likely cross-reactivity.
Target Information
AKT1 (PKB alpha) is a serine/threonine kinase involved in cell survival regulation. It inhibits apoptosis and promotes cell cycle progression by phosphorylating various targets.
Activated by insulin, PI3K, IGF1, and other growth factors, AKT1 plays a central role in cell survival signaling and is a major focus in cancer drug discovery.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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