Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Blue 660-40S, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Blue 660-40S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.4 µg)/test

Species Reactivity

Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Product Specifications

Additional Formats Available

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510, 530, 585, 610-70S, 660-120S, 760
• NovaFluor Red 685, 700, 725, 755
• NovaFluor Yellow 590, 610, 660, 690, 700, 730, 755
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Request custom conjugation available

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (Fc gamma RIII), which exists as two isoforms: FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, and NK cells.
• FcγRIIIB: Found on neutrophils (GPI-anchored form).

Expression patterns differ among primates; for example, CD16 is not found on neutrophils in macaques and baboons but is detected in sooty mangabeys.
Binding of IgG activates ADCC, phagocytosis, cytokine release, and antigen presentation.

3G8 recognizes the same epitope as CB16 but differs from B73.1 clone.
Each product includes one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells (5 µL (0.4 µg)/test).
Cell number: 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 509 nm
Emission: 665 nm
Laser: 488 nm (Blue)

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance and cell-mediated cytotoxicity.
The gene (FCGR3A) is similar to FCGR3B on chromosome 1.

• FCGR3A: Expressed on NK cells (transmembrane anchored).
• FCGR3B: Expressed on neutrophils (PI-anchored).

Mutations are linked to immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and neonatal neutropenia.
Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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