Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 660, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Red 660, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 4 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510 / 530 / 585 / 610-70S / 660-120S / 660-40S / 760
• NovaFluor Red 685 / 700 / 725 / 755
• NovaFluor Yellow 590 / 610 / 660 / 690 / 700 / 730 / 755
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (Fc gamma RIII).
CD16 exists as two isoforms: FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, NK cells
• FcγRIIIB: Expressed on neutrophils (GPI-anchored form)

Expression patterns are similar in non-human primates, though neutrophil expression varies among species.
Binding of IgG activates signal transduction pathways leading to ADCC, phagocytosis, cytokine release, and antigen presentation.

Epitope Recognition:
3G8 recognizes the same epitope as CB16 but distinct from B73.1.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells
Use at 4 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

Recommended Reagent:
Use CellBlox Blocking Buffer with NovaFluor conjugates.
Add 5 µL per sample or per 100 µL cell sample (10³–10⁸ cells).

Excitation: 637 nm
Emission: 659 nm
Laser: 633–640 nm (Red)

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures to minimize spillover and spectral spread.

Target Information

CD16 encodes a receptor that binds the Fc portion of IgG, mediating immune complex clearance and cellular cytotoxicity.

• Gene: FCGR3A (chromosome 1)
• Expression: NK cells (transmembrane), neutrophils (PI linkage via FCGR3B)
• Associated Diseases: Immunodeficiency 20, Herpes Zoster, Systemic Lupus Erythematosus, Alloimmune Neonatal Neutropenia
• Function: ADCC, phagocytosis, cytokine release, antigen presentation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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