Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Blue 610-70S, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Species Reactivity

Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Product Specifications

Additional Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 530
• NovaFluor Blue 585
• NovaFluor Blue 660-120S
• NovaFluor Blue 660-40S
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (Fc gamma RIII).
CD16 exists as two isoforms: FcγRIIIA and FcγRIIIB.

• FcγRIIIA: expressed on monocytes, macrophages, and NK cells
• FcγRIIIB: detected on neutrophils

Binding of IgG activates signal transduction pathways leading to ADCC, phagocytosis, cytokine release, and antigen presentation.

Based on cross-blocking studies, 3G8 recognizes the same epitope as CB16 but distinct from B73.1.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted, tested on human peripheral blood cells at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

CellBlox Plus Blocking Buffer:
Required for optimal staining with NovaFluor conjugates.
Add 5 µL per sample or per 100 µL cell sample (10³–10⁸ cells).
Cat. No. C001T06F01.

NovaFluor Technology:
Phiton-based nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spectral spread.

Excitation: 509 nm
Emission: 614 nm
Laser: 488 nm (Blue)

Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance, ADCC, and viral infection enhancement.
The gene FCGR3A (chromosome 1) is expressed on NK cells as a transmembrane glycoprotein, while FCGR3B is expressed on neutrophils via PI linkage.
Mutations are associated with Immunodeficiency 20, Herpes Zoster, and susceptibility to lupus and recurrent viral infections.
Alternative splicing produces multiple isoforms.

For Research Use Only.
Not for diagnostic procedures or resale without authorization.

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