Thermo Fisher Scientific J Chain Monoclonal Antibody (Mc19-9)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:200

-

ELISA (ELISA)

5 µg/mL

-

Immunoprecipitation (IP)

20 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Mc19-9

Immunogen

Human J Chain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_934333

Product Specific Information

This antibody does not detect J chain in intact IgM in ELISA Assays due to steric hindrance. In Western Blotting techniques MA1-80527 detects J chain in IgM only under reducing conditions.

Mouse anti Human J chain antibody, clone Mc19-9 reacts with human J chain as tested by ELISA using purified human Ig fragments as the solid phase target antigen.

Target Information

J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.