Thermo Fisher Scientific Phospho-mTOR (Ser2448) Polyclonal Antibody
Phospho-mTOR (Ser2448) 폴리클로날 항체로 인간 및 생쥐 시료에 반응. Western blot과 IHC(P)에서 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 세포 성장 및 증식 연구에 유용. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human mTOR (phospho Ser2448) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792875 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Sheep (100%).
- Positive Control: NIH-3T3 (starvation for 16 hr), NIH-3T3 (starvation for 16 hr and 200 ng/mL EGF treatment for 0.5 hr), 293T, 293T (200 ng/mL EGF treatment for 0.5 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with various forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are used as immunosuppressants and cancer therapeutics. Mutations in this gene can result in Smith-Kingsmore syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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