Thermo Fisher Scientific Phospho-mTOR (Ser2448) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human mTOR (phospho Ser2448). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:mTOR,uniProtId:P42345-1,ncbiNodeId:9606,antigenRange:2448,antigenLength:2549,antigenImageFileName:PA5-85736_mTOR_P42345-1_Rabbit.svg,antigenImageFileNamePDP:PA5-85736_mTOR_P42345-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2792875

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (100%), Rat (100%), Sheep (100%).

Positive Control: NIH-3T3 (starvation for 16 hr), NIH-3T3 (starvation for 16 hr and 200 ng/mL EGF treatment for 0.5 hr), 293T, 293T (200 ng/mL EGF treatment for 0.5 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently used as immunosuppressants and cancer therapeutics. Mutations affecting the gene results in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.