Thermo Fisher Scientific GAL4 Monoclonal Antibody (3G152)
GAL4 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3G152)로, WB, ICC/IF, ELISA, IP 등 다양한 분석에 사용 가능. 비결합형 액상 형태로 제공되며, 장기 보관 시 -20°C에서 안정적. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 0.1–1 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G152 |
| Immunogen | Recombinant fragment corresponding to amino acids 1–147 of S. cerevisiae GAL4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1958032 |
Product Specific Information
MA1-91587 detects GAL4 from yeast samples.
Target Information
GAL4 is a transcriptional activator that regulates genes involved in galactose catabolism.
The DNA-binding domain resides within the first 74 amino acids and includes a zinc finger motif. Amino acids 1–147 bind DNA in vitro, with GAL4 (1–74) showing specific but lower affinity compared to GAL4 (1–147).
GAL4 (1–147) binds to a specific 17 bp nucleotide sequence but does not activate transcription alone. Activation requires one of two acidic activation regions:
- Activating region I (amino acids 148–238)
- Activating region II (amino acids 768–881)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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