Thermo Fisher Scientific GAL4 Monoclonal Antibody (3G152)

Applications

Tested Dilution

Publications

Western Blot (WB)

3 µg/mL

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3G152

Immunogen

Recombinant fragment, corresponding to amino acids 1-147 of S. cerevisiae GAL4.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1958032

Product Specific Information

MA1-91587 detects GAL4 from yeast samples.

Target Information

GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.