Thermo Fisher Scientific Tyrosinase Recombinant Rabbit Monoclonal Antibody (JA52-11)
Tyrosinase 단백질을 인식하는 재조합 토끼 단클론 항체입니다. Western blot, IHC(P), ICC/IF에 사용 가능하며 높은 특이성과 재현성을 제공합니다. Protein A로 정제된 액상 형태로, 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA52-11 |
| Immunogen | Recombinant protein within Human Tyrosinase (aa 1–340) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809942 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and expressed, followed by screening for optimal clones.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The tyrosinase enzyme catalyzes key steps in the conversion of tyrosine to melanin. It possesses both tyrosine hydroxylase and dopa oxidase activities and requires copper for function.
Mutations in the TYR gene can cause oculocutaneous albinism, while polymorphisms influence skin pigmentation variation.
A pseudogene similar to the 3′ half of this gene exists in the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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