Thermo Fisher Scientific Phosphotyrosine Recombinant Rabbit Monoclonal Antibody (JA10-49)
Recombinant rabbit monoclonal antibody recognizing phosphorylated tyrosine residues. High specificity and sensitivity with consistent lot performance. Suitable for WB, ICC/IF, and Flow Cytometry. Provided in liquid form, unconjugated, for research use ...
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA10-49 |
| Immunogen | CXXXX-pY-XXXXNH2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809944 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits, followed by screening individual clones to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulations
- Broader immunoreactivity to diverse targets due to the larger rabbit immune repertoire
Target Information
Tyrosine phosphorylation plays a critical role in signal transduction from transmembrane receptors and oncogene tyrosine kinases. Phosphorylation of specific tyrosine residues is a key mechanism in normal mitogenesis, cell cycle progression, and oncogenic transformation.
Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable tools for studying tyrosine-phosphorylated proteins and their biochemical pathways. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is particularly useful for detecting P-Tyr proteins in immunohistochemical and immunocytochemical protocols, especially when secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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