Thermo Fisher Scientific ADFP Recombinant Rabbit Monoclonal Antibody (JA31-81)
Recombinant rabbit monoclonal antibody recognizing ADFP (Adipophilin). Validated for WB, ICC/IF, and Flow Cytometry. High specificity and sensitivity with consistent performance. Suitable for human, mouse, and rat samples. For research use only.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JA31-81 |
| Immunogen | Synthetic peptide within Human ADFP (aa 18–67) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at −20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809941 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Adipocyte differentiation-related protein (ADFP, also known as Adipophilin) is a major component of milk lipid globule surface membranes in humans, cows, and rats. It is part of a detergent-insoluble complex containing butyrophilin and xanthine oxidase.
ADFP is expressed in various cultured cell lines (fibroblasts, endothelial, epithelial cells) and specific tissue cell types such as lactating mammary epithelial cells, adrenal cortex cells, Sertoli and Leydig cells, and hepatocytes in fatty liver conditions.
ADFP serves as a potential marker for identifying specialized differentiated cells containing lipid droplets and for studying diseases associated with fat-accumulating cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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