Thermo Fisher Scientific CD9 Monoclonal Antibody (eBioKMC8 (KMC8)), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats:

• Unconjugated
• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• PE
• Request custom conjugation

Product Specific Information

The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily characterized by four hydrophobic domains spanning the cell membrane and short cytoplasmic domains. CD9 is expressed by multiple cell types including monocytes, macrophages, platelets, B cells, T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts, and neuroblasts. It functions as a co-stimulatory molecule on naive T cells and is essential for gamete fusion in oocytes.

In macrophages, CD9 associates with FcγRs to modulate phagocytosis and inflammatory signaling. In B cells, it serves as a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, CD9 facilitates the association of heterologous MHC II molecules. Expression levels vary among donors.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested on mouse bone marrow cells at ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited by the violet laser (405 nm) and emits at 600 nm. Recommended filter: 610/20 bandpass. Ensure instrument compatibility.

Staining Recommendations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark with minimal effect on brightness and FRET efficiency.

Excitation / Emission / Laser:
405 nm / 600 nm / Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD9 antigen is a glycoprotein expressed on developing B lymphocytes, platelets, monocytes, eosinophils, basophils, stimulated T lymphocytes, neurons, and glial cells. It belongs to the tetraspanin family, which spans the membrane four times. CD9 regulates cell activation and aggregation, potentially through interaction with integrin CD41/CD61 (GPIIb/GPIIIa). It is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion. CD9 also plays roles in adhesion, proliferation, and suppression of cancer cell metastasis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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