Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 737
• Brilliant Violet 605
• Brilliant Violet 786
• eFluor 450
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor and member of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation via BCR complex, CD40, or cytokine receptors. It is also expressed by activated T cells and peritoneal cells.

CD86 interacts with T cell surface molecules CD28 and CTLA-4, playing a critical role in T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The upregulation kinetics of CD86 suggest its major contribution during the primary immune response phase.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes. Use ≤0.125 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended filters: 685 LP mirror and 710/20 bandpass filter.

Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser

Target Information

CD86 is one of two ligands (along with CD80) for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation through CD86/CD80 can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells. It binds CD28 (activating signal) and CTLA-4 (inhibitory signal), regulating T-cell activation and immune responses. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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