Thermo Fisher Scientific CD86 (B7-2) Monoclonal Antibody (GL1), Alexa Fluor 700, eBioscience
마우스 CD86(B7-2)을 인식하는 GL1 단클론 항체로, Alexa Fluor 700 형광 표지. Flow cytometry에 최적화되어 있으며, 면역세포 활성화 연구에 활용 가능. 696/719 nm 파장 특성으로 적색 레이저 검출에 적합. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | View 99 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GL1 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848464 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 605
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Product Specific Information
The GL1 monoclonal antibody reacts with mouse CD86 (B7-2), an ~80 kDa surface receptor and member of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells, and is upregulated upon stimulation via BCR complex, CD40, or cytokine receptors. It is also expressed by activated T cells and peritoneal cells.
CD86 interacts with T cell surface molecules CD28 and CTLA-4, playing a critical role in T-B cell crosstalk, T cell costimulation, autoantibody production, and Th2-mediated Ig production. The upregulation kinetics of CD86 suggest its major contribution during the primary immune response phase.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes. Use ≤0.125 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Recommended filters: 685 LP mirror and 710/20 bandpass filter.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red Laser
Target Information
CD86 is one of two ligands (along with CD80) for CTLA4 and CD28, acting as a costimulatory molecule in T-cell activation during antigen presentation. Insufficient co-stimulation through CD86/CD80 can induce tolerance. CD86 is a type I membrane protein of the immunoglobulin superfamily, expressed by antigen-presenting cells. It binds CD28 (activating signal) and CTLA-4 (inhibitory signal), regulating T-cell activation and immune responses. Alternative splicing produces multiple isoforms. CD86 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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