Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Alexa Fluor 700, eBioscience
CD71(Transferrin Receptor) 인식 OKT9 단클론 항체로, Alexa Fluor 700 형광 표지된 제품입니다. 인간 세포에 반응하며 Flow Cytometry 분석에 최적화되어 있습니다. 세포 성장 및 철분 대사 연구용으로 사용됩니다.
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Thermo Fisher Scientific CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 10 publications |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT9 (OKT-9) |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815242 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The OKT9 monoclonal antibody reacts with human CD71, a 170–180 kDa type II transmembrane protein known as the transferrin receptor. CD71 exists as a homodimer on the cell surface and is essential for cellular growth. It is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates CD71 expression.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry using stimulated normal human peripheral blood cells. Recommended usage is 5 µL (0.25 µg) per test (final volume 100 µL).
Cell number may vary between 10⁵–10⁸ cells/test.
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and can be excited with the red laser (633–647 nm). Most instruments require a 685 LP mirror and 710/20 band pass filter.
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
Target Information
Cellular iron uptake occurs via receptor-mediated endocytosis of ligand-bound transferrin receptor into endosomes. Acidification releases iron, and the apotransferrin-receptor complex recycles to the cell surface. The transferrin receptor is crucial for erythrocyte and nervous system development. Serum transferrin receptor (sTfR) is also used to detect erythropoietin (EPO) misuse and diagnose anemia related to conditions such as rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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