Thermo Fisher Scientific CD44v4 Monoclonal Antibody (CD44v4, 1219)
인간 CD44v4 단백질을 인식하는 mouse monoclonal 항체. IHC(P) 실험에 적합하며, 세포 간 상호작용 및 이동 연구에 활용 가능. Protein A/G 정제, 200 µg/mL 농도, 4°C 보관.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD44v4, 1219 |
| Immunogen | Recombinant fragment corresponding to the v4 domain of human CD44 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD44 cell surface antigen is a 100 kDa glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 functions as a receptor for hyaluronic acid (HA) and is involved in cell–cell interactions, adhesion, and migration. It also participates in lymphocyte activation, recirculation, and homing.
CD44 expression may be upregulated in some carcinomas and is speculated to be associated with metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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