Thermo Fisher Scientific CD44v4 Monoclonal Antibody (CD44v4, 1219)
인간 CD44v4 항원을 인식하는 Mouse IgG2a 단일클론 항체. IHC(P)에서 1–2 µg/mL로 사용 가능하며, 세포 접착 및 이동 연구에 적합. 단백질 A/G로 정제된 액상 형태로 4°C에서 보관.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD44v4, 1219 |
| Immunogen | Recombinant fragment corresponding to the v4 domain of human CD44 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD44 cell surface antigen is a 100 kDa glycoprotein widely expressed on human leukocytes, white matter of the brain, and some epithelial cells of the intestine and breast.
Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues.
CD44 acts as a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, adhesion, and migration.
It participates in various cellular functions including lymphocyte activation, recirculation, and homing.
CD44 expression may be up-regulated in certain carcinomas, potentially related to metastatic potential.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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