Thermo Fisher Scientific NOV Polyclonal Antibody
인간 NOV/CCN3 단백질을 인식하는 염소 유래 폴리클로날 항체. Western blot, IHC, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제. 동결건조 형태로 제공되며 -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL |
| ELISA (Detection) | 0.1–0.4 µg/mL |
| ELISA (Capture) | 0.2–0.8 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human NOV/CCN3 (Thr32–Met357) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608679 |
Product Specific Information
- In sandwich ELISAs, approximately 7% cross-reactivity with recombinant mouse NOV/CCN3 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
NOV is a member of the CCN family of secreted, cysteine-rich regulatory proteins. The full-length NOV protein contains four structural domains that confer distinct, and sometimes opposing, biological activities. Elevated expression of NOV is associated with certain tumors, including Wilms' tumor and most nephroblastomas. However, in other tumor types and certain cancer cell lines, increased tumorigenicity and proliferation are correlated with decreased NOV expression. Additionally, NOV induces cell adhesion and migration by signaling through specific cell-surface integrins and by binding to heparin sulfate proteoglycans and fibulin 1C. NOV has also been reported to exert proangiogenic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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