Thermo Fisher Scientific PI3K p85 alpha Monoclonal Antibody (8-2D-4D)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

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Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

8-2D-4D

Immunogen

Purified fragment (aa 333 - 430) of recombinant human PI-3-K p85 alpha subunit. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85 alpha,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:333-430,antigenLength:724,antigenImageFileName:MA1-21830_PI3K_p85_alpha_P27986-1_House_mouse.svg,antigenImageFileNamePDP:MA1-21830_PI3K_p85_alpha_P27986-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560620

Product Specific Information

Recommended positive controls: Jurkat or A431 cells.

By Western Blot this antibody detects a band of 85kDa, which corresponds to the predicted molecular weight of PI 3 kinase.

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.