Thermo Fisher Scientific Prom1 Monoclonal Antibody (2F8C5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:100

-

Immunocytochemistry (ICC/IF)

1:100-1:200

View 1 publication 1 publication

In vitro Assay (IV)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F8C5

Immunogen

Purified recombinant fragment of human Prom1 (AA:20-108) expressed in E.Coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD133 (Prominin-1),uniProtId:O43490-1,ncbiNodeId:9606,antigenRange:20-108,antigenLength:865,antigenImageFileName:MA1-219_CD133_Prominin-1_O43490-1_House_mouse.svg,antigenImageFileNamePDP:MA1-219_CD133_Prominin-1_O43490-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2725113

Product Specific Information

MA1-219 Prom1 antibody detects Prom1 (CD133) by western blot and immunofluorescence.
MA1-219 Prom1 antibody detects Prom1 by western blot at ~130 kDa.

Target Information

The CD133 (AC133) antigen is a 97 kDa glycoprotein, with 5 transmembrane domains, that shows up as a 120 kDa band on protein gels due to glycosylation. CD133 is a cell surface marker expressed by immature hematopoietic stem cells but not mature blood cells. In addition to hematopoietic stem and progenitor cells, CD133 has been found to be a marker for other stem and progenitor cells including neural and embryonic stem cells. CD133 has been shown to be expressed in cancers, including some leukemias and brain tumors, that may be derived from stem cells. Low level expression could also be detected in the kidney, pancreas, placenta, and fetal liver. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of the CD133 gene is also associated with several types of cancer. CD133 is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner, and multiple transcript variants encoding different isoforms have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.