Thermo Fisher Scientific AMPK gamma-1 Monoclonal Antibody (7H3E9)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

-

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Flow Cytometry (Flow)

1:200-1:400

-

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

7H3E9

Immunogen

Purified recombinant fragment of human PRKAG1 (AA: 230-331) expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMPK gamma-1,uniProtId:P54619-1,ncbiNodeId:9606,antigenRange:230-331,antigenLength:331,antigenImageFileName:MA5-31866_AMPK_gamma-1_P54619-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31866_AMPK_gamma-1_P54619-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2787489

Product Specific Information

MA5-31866 has been tested in indirect ELISA.

Target Information

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.