Thermo Fisher Scientific AMPK gamma-1 Monoclonal Antibody (7H3E9)
AMPK gamma-1 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 및 ELISA에 적합합니다. 인간 시료에 반응하며, Protein G로 정제된 액상 형태입니다. 에너지 대사 연구 및 AMPK 신호전달 분석에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7H3E9 |
| Immunogen | Purified recombinant fragment of human PRKAG1 (AA: 230–331) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787489 |
Product Specific Information
MA5-31866 has been tested in indirect ELISA.
Target Information
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It functions as an energy-sensing enzyme that monitors cellular energy status. Upon activation by metabolic stress, AMPK phosphorylates and inactivates key enzymes such as acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), which are involved in fatty acid and cholesterol biosynthesis. This subunit represents one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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