Thermo Fisher Scientific MAPK1 Monoclonal Antibody (OTI4D10), TrueMAB
MAPK1(ERK2)를 인식하는 Mouse monoclonal antibody로 Western blot 및 Immunoprecipitation에 적합. Dog, Human, Rat 반응성. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 MAPK signaling 관련 단백질 분석에 활용 가능.
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunoprecipitation (IP) | 2–4 µg/mg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4D10 |
| Immunogen | Full length human recombinant protein of human MAPK1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK1 (ERK2) is a serine/threonine kinase that activates p90 RSK, MSK, ELK1, and Stat3, and plays a key role in the MAPK pathway.
MAP kinases (ERKs) integrate multiple biochemical signals and regulate processes such as proliferation, differentiation, transcription, and development.
Activation requires phosphorylation by upstream kinases, after which MAPK1 translocates to the nucleus to phosphorylate nuclear targets.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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