Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 450, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), eFluor 450, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 506 / 570 / 615 / 660
• FITC
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein, which exists in two isoforms (345 and 395 kDa) expressed in dividing cells. Ki-67 is present during active phases of the cell cycle (G1, S, G2, and mitosis) and absent in resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
In tumor studies, Ki-67 expression serves as a marker for determining the proliferating cell fraction.

This antibody also recognizes canine Ki-67.

Applications Reported:

• Intracellular staining using Foxp3 Staining Buffers followed by flow cytometric analysis.

Applications Tested:

• Pre-titrated and validated with Foxp3/Transcription Factor Buffers Set (Product #00-5523-00).
• Recommended use at 5 µL (0.06 µg) per test (100 µL final volume).
• Suitable for 10⁵–10⁸ cells/test (empirical determination required).

Fluorochrome Information:

• eFluor® 450 is an alternative to Pacific Blue®.
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet.
• Post-manufacturing filtration: 0.2 µm.

Target Information

Ki-67 is a nuclear protein expressed during cell cycle phases G1, S, G2, and M. It contains a forkhead-associated (FHA) domain and associates with euchromatin, centromeric heterochromatin, and nucleolus.
Expression pattern:

• G1: Perinucleolar
• G2: Nuclear matrix
• M phase: Chromosomal localization

Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases from G1 through mitosis. It helps determine the growth fraction in neoplasms, correlating with tumor grade.
Also utilized as a neuronal marker for cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지