Thermo Fisher Scientific Phospho-IRS1 (Tyr989) Polyclonal Antibody
IRS1 단백질의 Tyr989 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot에 최적화되어 있으며 Human, Mouse, Rat 반응성 확인됨. 고순도 정제 및 안정적 액상 형태로 -20°C 보관. 연구용 인산화 신호 전달 연구에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500–1:2,000
View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human IRS1 around the phosphorylation site of Tyr989 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899229 |
Product Specific Information
Antibody detects endogenous levels of IRS1 only when phosphorylated at Tyr989.
Target Information
IRS-1 is a major substrate of the insulin receptor and becomes phosphorylated upon stimulation by insulin, IGF-1, and IL-4. Phosphorylation occurs on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates part of the insulin-stimulated serine/threonine phosphorylation of IRS-1, with Thr502 and Ser99 identified as major phosphorylation sites in rat IRS-1. IRS-1 also contains three potential 14-3-3 binding sites (Ser270, Ser374, Ser641), with the Ser270 motif located within the phosphotyrosine binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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