Thermo Fisher Scientific Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 615, eBioscience

Applications

Tested Dilution

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Isotype Control

Clone

eBM2a

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 647
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853516

Product Specific Information

Description: This is a monoclonal mouse IgG2a antibody. It is used as an isotype control for mouse IgG2a antibodies.

Applications Reported: This eBM2a antibody has been reported for use in immunohistochemical staining and immunocytochemistry.

Applications Tested: This eBM2a antibody has been tested by immunocytochemistry and immunohistochemical staining. Use isotype control at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.