Thermo Fisher Scientific Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 615, eBioscience
Mouse IgG1 kappa 동종 대조 항체로, eFluor 615 형광 표지. IHC(P) 및 ICC/IF에 사용 가능하며, 실험 항체와 동일 농도로 적용. 595/614 nm 파장에서 최적 발광. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 20 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Control (Ctrl) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | P3.6.2.8.1 |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% Sodium azide |
| Storage Conditions | 4°C, store in dark, Do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10733160 |
Available Formats
- Unconjugated
- Alexa Fluor™ 488, 532, 561, 647, 660, 700
- APC, APC-eFluor™ 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421–786 series
- eFluor™ 450, 506, 570, 660
- FITC
- Functional Grade
- Pacific Orange
- PE and PE conjugates (Cyanine5, 5.5, 7, eFluor 610)
- PerCP conjugates (Cyanine5.5, eFluor 710)
- Super Bright 436–780 series
- Custom conjugation available
Product Specific Information
The monoclonal Mouse IgG1 kappa immunoglobulin serves as an isotype control.
It has been reported for use in immunocytochemistry (ICC) and immunohistochemistry (IHC-P) applications.
This antibody has been tested on fixed and permeabilized MDCK cells and formalin-fixed paraffin-embedded human brain tissue.
Use the isotype control at the same concentration as the experimental antibody.
Filter Recommendation:
For eFluor™ 615 conjugate, use a filter capturing the 615 nm emission wavelength. A standard Alexa Fluor™ 594 filter is acceptable.
Excitation / Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Mouse IgG1 is a subtype of immunoglobulin G produced by B cells in response to antigen exposure.
It is the most abundant IgG subclass in serum and plays a key role in adaptive immunity, including:
- Opsonization
- Complement activation
- Antibody-dependent cell-mediated cytotoxicity (ADCC)
- Regulation of T and B cell activation
Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry, and also in therapeutic development such as immunotherapy and vaccine research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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