Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1B4), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, WB, IHC, ICC/IF 등 다양한 실험에 사용 가능. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼, -20°C 보관. 연구용으로 세포 성장 및 신호전달 경로 연구에 적합.
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Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1B4), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B4 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase that functions in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx.
Phosphorylation at serine 218 and 222 residues by Raf1 activates MEK1.
MAP2K1 is involved in stress response signaling, apoptosis, and cytokine-induced proliferation.
Mutations in this gene can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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