Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI5D3), TrueMAB
MAP2K1(MEK1) 단백질을 표적하는 단클론 항체로, Western blot, IHC, ICC/IF에 적합. 인간 시료 반응성, Mouse IgG2b 아이소타입. 동결건조 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 연구용으로 세포 성장 및 신호전달 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:8000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D3 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in MAPK cascades that regulate cell growth and differentiation.
Activated by growth factors, cytokines, and calcium influx, MEK1 is phosphorylated at serine 218 and 222 by Raf1.
Involved in stress response signaling, apoptosis, and cytokine-induced proliferation.
Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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