Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1F9), TrueMAB
MAP2K1 단백질을 표적하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 마우스, 랫, 개 반응성이 있으며, 라이오필라이즈드 형태로 제공됩니다. 연구용으로 세포 성장 및 신호전달 연구에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:4000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F9 |
| Immunogen | Full length human recombinant protein of human MAP2K1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional round of desalting (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, and membrane-dependent depolarization. Phosphorylation of serine residues 218 and 222 by Raf1 activates Mek1. It is involved in stress response signaling, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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