Thermo Fisher Scientific RHBDD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RHBDD1. Recombinant protein control fragment (Product #RP-90982). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RHBDD1,uniProtId:Q8TEB9-1,ncbiNodeId:9606,antigenRange:209-313,antigenLength:315,antigenImageFileName:PA5-53102_RHBDD1_Q8TEB9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-53102_RHBDD1_Q8TEB9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2646524

Product Specific Information

Immunogen sequence: PLKKIMEACA GGFSSSVGYP GRQYYFNSSG SSGYQDYYPH GRPDHYEEAP RNYDTYTAGL SEEEQLERAL QASLWDRGNT RNSPPPYGFH LSPEEMRRQR LHRFD

Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 73%.

Target Information

The Rhomboid family of proteins is made up of several widely conserved polytopic membrane serine proteases that play roles in growth and development. RHBDD1 is highly expressed in the testis and is involved in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. Overexpression or suppression by RNAi of RHBDD1 in 293 cells will reduce or enhance BIK-mediated apoptosis, respectively, demonstrating that RHBDD1 modulates BIK-mediated apoptotic activity. In GC-1 cells, a spermatogonia cell line that can differentiate into spermatids within the seminiferous tubules, suppression of RHBDD1 expression by RNAi caused the cells to lose the ability to survive and differentiate in mouse seminiferous tubules, suggesting that RHBDD1 may be associated with mammalian spermatogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.