Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Alexa Fluor 700, eBioscience
인간 CD3 복합체의 epsilon 서브유닛을 인식하는 OKT3 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. 유세포분석에 최적화되어 있으며, T세포 활성화 연구에 적합합니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | 33 |
| Functional Assay | - | 1 |
| Immunomicroscopy (IM) | - | 1 |
| T-Cell Activation (TCA) | - | 5 |
| Miscellaneous (PubMed) | - | 2 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696/719 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10714978 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Custom conjugation available
Product Specific Information
The OKT3 monoclonal antibody binds to an epitope on the epsilon-subunit of the human CD3 complex. OKT3 has potent immunosuppressive properties and has been effective in treating renal, heart, and liver allograft rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly, trafficking, and surface expression. CD3 is expressed by thymocytes during development and by all mature T cells.
Crosslinking of TCR initiates intracellular signaling, leading to activation and proliferation.
This antibody is validated for flow cytometric analysis and has been pre-titrated at 5 µL (0.5 µg) per test. A test is defined as the amount of antibody required to stain cells in a 100 µL volume (typically 10⁵–10⁸ cells/test).
Alexa Fluor® 700 emits at 723 nm and is excited by the red laser (633 nm). Recommended optics: 685 LP mirror and 710/20 filter.
Excitation: 633–647 nm
Emission: 723 nm
Laser: Red
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD3 subunit complex transduces antigen-recognition signals in T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on chromosome 11. These subunits associate with cytoplasmic signaling molecules via tyrosine-based motifs.
CD3 is present on 68–82% of peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells, but not on B or NK cells. Reduced T lymphocyte percentages may occur in autoimmune diseases. Defects in CD3 genes are linked to immunodeficiency.
For Research Use Only. Not for diagnostic or therapeutic use.
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