Thermo Fisher Scientific CD44 Monoclonal Antibody (IM7), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–737
• Brilliant Violet 421, 650, 711
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue, Red, Yellow, Violet, UV series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436–780
• Request custom conjugation

Product Specific Information

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen, and peripheral B and T cells can upregulate CD44 expression. CD44 functions as an adhesion molecule through binding to hyaluronate, an extracellular matrix component.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤0.5 µg/test)
• Recommended: Titrate antibody for optimal performance

Brilliant Violet™ 605 (BV605) emits at 605 nm and is for cytometers with a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (e.g., Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

• Light Sensitivity: Protect vial and samples from light
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in dark
• Not Compatible: Methanol-based fixation
• Excitation: 407 nm
• Emission: 605 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD44 is a 100 kDa type 1 transmembrane glycoprotein expressed on human leukocytes, brain white matter, and various epithelial cells. It serves as a receptor for hyaluronic acid (HA), mediating cell-cell interactions, adhesion, and migration. CD44 plays roles in lymphocyte activation, recirculation, and homing. Expression may be upregulated in certain carcinomas, potentially linked to metastasis. Multiple isoforms arise from complex alternative splicing.

Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 may also relate to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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