Thermo Fisher Scientific CD38 Monoclonal Antibody (90), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD38 Monoclonal Antibody (Clone 90), Brilliant Violet 605

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor™ 700
• APC
• APC-eFluor™ 780
• Biotin
• Brilliant UV™ 395
• Brilliant Violet™ 480
• eFluor™ 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor™ 610
• PerCP-eFluor™ 710
• Custom conjugation available

Product Specific Information

The 90 monoclonal antibody reacts with mouse CD38, a ~42 kDa type II transmembrane protein. CD38 is expressed at varying levels on B cells throughout differentiation, as well as on thymocytes, mature T cells, and NK cells. Crosslinking of CD38 induces B-cell proliferation, enhanced by IL-4 and LPS co-signals. CD38 acts as a counter-receptor for CD31 and functions as an ectoenzyme with cyclase and hydrolase activity, playing roles in lymphocyte activation and differentiation.

• Reported Application: Flow cytometric analysis
• Tested Application: Flow cytometric analysis of mouse splenocytes
• Recommended Use: ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Optimization: Titrate for best assay performance

Fluorophore Information:
Brilliant Violet™ 605 (BV605) emits at 605 nm and is compatible with cytometers equipped with a violet (405 nm) laser. Protect from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

Fixation Guidance:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). BV605 is not compatible with methanol-based fixation.

Excitation / Emission / Laser:
407 nm / 605 nm / Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein involved in activation, proliferation, and differentiation of lymphocytes, and apoptosis of progenitor cells. It functions as an ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and cell surface receptor. CD38 antibodies are useful for lymphoma and leukemia subtyping, plasma cell detection (myeloma identification), and as markers for activated B/T cells. CD38 is expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions are associated with chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

제품 이미지