Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), Brilliant Violet 605, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.25 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 711
• eFluor 450
• FITC
• NovaFluor Blue / Red / UV / Violet / Yellow Series
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436 / 600 / 645 / 702 / 780)
• Custom conjugation available upon request

Product Specific Information

The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing a key role in T cell–B cell interactions.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells at 5 µL (0.25 µg)/test.

Brilliant Violet™ 605 (BV605) emits at 605 nm and is intended for use on violet (405 nm) laser-equipped cytometers. Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light.
Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, involved in regulation of lymphocyte proliferation and apoptosis. It is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral T cells, and a subpopulation of B cells. Soluble CD27 (sCD27) serves as a prognostic marker for B cell malignancies. CD27 interacts with CD70 and SIVA, mediating T-cell activation and apoptosis. Dysfunctions are associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for diagnostic or resale use.

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