Thermo Fisher Scientific Placental Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody (SP15)
인간 PLAP 단백질을 인식하는 토끼 리콤비넌트 단클론 항체로, IHC(P) 및 유세포분석에 적합. 단백질 A로 정제된 액상 항체이며, 4°C 보관 권장. 시험용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | - |
| Flow Cytometry (Flow) | 1:200 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Proprietary |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SP15 |
| Immunogen | Recombinant protein encoding human placental alkaline phosphatase |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.021 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537911 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to staining.
- Use 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
- After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
- Suggested positive control: seminoma or placenta tissue.
Target Information
Placental Alkaline Phosphatase (PLAP) plays an important role in regulating inflammatory disease processes. There are four related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney types. PLAP reacts with a membrane-bound isoenzyme (Regan and Nagao type) found in the placenta during the third trimester of gestation.
PLAP is useful for identifying testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
A proposed function of PLAP is matrix mineralization; however, mice lacking functional PLAP show normal skeletal development. PLAP has been linked to hypophosphatasia, a disorder characterized by hypercalcemia and skeletal defects. The severity and onset vary depending on the specific mutation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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