Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (53-6.7), Brilliant Ultra Violet 661, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 615, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule, a 32–34 kDa cell surface receptor expressed as CD8αβ or CD8αα dimers. CD8 binds MHC class I and associates with p56lck, playing a key role in T cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes at ≤1.0 µg/test (100 µL final volume). Cell count range: 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.

Fluorophore Information:
Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm, excitable at 355 nm UV laser. Ensure instrument compatibility.

Staining Panel Recommendation:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54).

Fluorophore Details:
Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8 engagement triggers Lck recruitment, leading to CTL activation and pathogen/tumor clearance. Differentiation of CD8+ T cells is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지