Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 661, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 610-30S, 660-40S
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b (integrin alphaM, 165–170 kDa). CD11b associates with CD18 to form the alphaMbeta2 integrin (Mac-1), which binds to CD54 (ICAM-1), C3bi, and fibrinogen. CD11b is expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells. M1/70 also cross-reacts with human CD11b and can detect this antigen on human monocytes, granulocytes, and NK cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: 0.25 µg/test (100 µL volume, 10⁵–10⁸ cells/test)
• Fluorochrome: Brilliant Ultra Violet™ 661 (BUV661), excitation 350 nm, emission 660 nm
• Laser: Ultraviolet (355 nm)

Note: Use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples may be stored up to 3 days at 4°C in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54).

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (integrin beta-2). This complex, known as Mac-1 or CR3, mediates binding to ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X. CD11b is expressed on monocytes/macrophages, granulocytes, NK cells, dendritic cells, and microglia. It is commonly used as a microglial marker and shows variable expression depending on macrophage tissue origin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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