Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 661, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Brilliant Ultra Violet 661, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking via UCHT1 induces cellular activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on human peripheral blood cells (5 µL/test, 0.5 µg/test, final volume 100 µL)
• Cell Count Range: 10⁵–10⁸ cells/test

Brilliant Ultra Violet™ 661 (BUV661) emits at 660 nm and is optimized for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies, apply Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize non-specific interactions.

• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples may be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness.

Excitation: 350 nm
Emission: 660 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals in T cells and regulates TCR expression. It includes CD3 gamma, delta, epsilon, and zeta subunits—members of the immunoglobulin superfamily encoded on chromosome 11. CD3 is expressed on 68–82% of peripheral lymphocytes and 65–85% of thymocytes but absent on B and NK cells. Defects in CD3 genes are associated with immunodeficiency.

For Research Use Only. Not for diagnostic use or resale.

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