Thermo Fisher Scientific CD278 (ICOS) Monoclonal Antibody (ISA-3), PE-Cyanine5.5, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Red 660
• NovaFluor Red 710
• NovaFluor Yellow 700
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1, and AILIM. ICOS is a T cell-specific activation molecule and a member of the CD28/CTLA-4 family. It plays a crucial role in T cell activation and humoral immune responses, particularly in memory B cell and plasma cell generation.

ICOS binds to its ligand B7h/B7RP-1 on activated antigen-presenting cells and inflamed tissues. Plate-bound ISA-3 induces T cell costimulation and cytokine production (IL-4, IL-5, IL-10) but not IL-2. The clone C398.4A (anti-mouse ICOS/H4) cross-reacts with human ICOS but binds a different epitope.

For brightest staining of ICOS on activated human T cells, use Product #13-9948-82 or Product #12-9948-42.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on stimulated human peripheral blood cells. Use 5 µL (0.25 µg) per test (100 µL total volume).
Light Sensitivity: Protect from light due to photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) for up to 3 days at 4°C in the dark.

Excitation: 488–561 nm
Emission: 695 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers

Target Information

ICOS (CD278) encodes a protein of the CD28/CTLA-4 receptor family. It functions as a homodimer and is essential for cell-cell signaling, immune response regulation, and cell proliferation. ICOS is associated with disorders such as Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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