Thermo Fisher Scientific CD85j (ILT2) Monoclonal Antibody (HP-F1), Super Bright 600, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Recommended Dilution

• 5 µL (0.5 µg)/test

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Product Specifications

Available Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 737
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 700
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 590
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1.
CD85j is a member of the ILT/LIR/MIR receptor family, a single transmembrane glycoprotein with four ITIMs that signal via SHP-1.
It is expressed on myeloid cells (monocytes, dendritic cells) and subsets of NK, T, and B lymphocytes. Expression correlates with leukemias (ALL, CLL) and viral infections (HIV, EBV, CMV).
Ligands include MHC Class I molecules such as HLA-G, A, F, B27, E, and F.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for normal human peripheral blood cells
• Recommended Use: 5 µL (0.5 µg) per test (final volume 100 µL; 10⁵–10⁸ cells/test)

Super Bright 600 is a tandem dye excited by the 405 nm violet laser and emits at 600 nm.
Recommended detection filter: 610/20 bandpass. Ensure instrument compatibility.

When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark.

Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

This gene belongs to the leukocyte immunoglobulin-like receptor (LIR) family, located at chromosome 19q13.4.
The encoded protein is part of the subfamily B class of LIR receptors, containing extracellular immunoglobulin domains, a transmembrane domain, and cytoplasmic ITIM motifs.
It is expressed on immune cells, binds MHC class I molecules on antigen-presenting cells, and transduces inhibitory signals to regulate immune responses and prevent autoreactivity.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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