Thermo Fisher Scientific Cas9 Monoclonal Antibody (10C11-A12)
Cas9 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 10C11-A12). Western blot, ICC/IF, Flow cytometry 등 다양한 응용에 사용 가능. Streptococcus pyogenes Cas9에 반응하며, 연구용으로 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:200–1:500
- View publication: 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria |
| Published Species | Bacteria |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C11-A12 |
| Immunogen | Cas9 nuclease |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2633310 |
Product Specific Information
Cas9 (10C11-A12) Mouse mAb recognizes transfected levels of total Cas9 protein.
Stable Cas9 U2OS cell line used in validation experiments expresses Cas9 at lower levels.
Assay results may vary depending on expression level of Cas9 in cell lines tested.
This product was tested in Streptococcus pyogenes bacteria.
Target Information
Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in bacteria and genetic engineering.
This system uses RNA to direct the nuclease to specific DNA sequences, enabling:
- Indel mutations
- Sequence replacements or insertions
- Large deletions or genomic rearrangements
- Upregulation of endogenous genes
- Alteration of histone modifications or DNA methylation
Cas9 variants:
- SpCas9: Derived from Streptococcus pyogenes
- SaCas9: Derived from Staphylococcus aureus, smaller size (1053 AA vs. 1368 AA), high efficiency, nickase activity, and specificity.
Recognizes NNGRRT PAM and performs DNA cleavage with spacer lengths of 20–24 nucleotides.
Suitable for all-in-one delivery of Cas9 and multiple gRNA cassettes using AAV vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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