Thermo Fisher Scientific CDK4 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Sheep, Bovine, and Pig

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 belongs to the cyclin-dependent protein kinase family and regulates cell proliferation during the G1 phase of the cell cycle. It forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 mediates phosphorylation of the C-terminal region of Rb protein, leading to transcriptional repression of the E2F complex. CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major substrate of CDK4 and CDK2. Although CDK6 and CDK4 both phosphorylate Rb protein, they show different residue selectivity in vitro—CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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