Thermo Fisher Scientific Salmonella LPS Monoclonal Antibody (A99H)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:10-1:50

-

ELISA (ELISA)

1:20-1:200

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

A99H

Immunogen

Salmonellae sp. native protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539632

Product Specific Information

This antibody is specific for common LPS core of all Salmonellae O-serogroups tested: A, B, C1, C2, D, E1, E3, E4, F, G1 and G2. This does not cross-react with E. coli (mix), Klebsiella, Citrobacter, Pseudomonas, Yersinia, Shigella, Proteus or Legionella.

Purity is >90%.

Recommended pairs for ELISA: MA5-18258 (capture) and MA5-18259 or MA5-18260 (conjugated, detection).

Target Information

The genus Salmonella is a member of the family Enterobacteriaceae. The genus is composed of Gram negative bacilli that are facultative and flagellated (motile). Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae). Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria. This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A. Endotoxins evoke fever and can activate complement, kinin and clotting factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.