Thermo Fisher Scientific NUMB Polyclonal Antibody
NUMB 단백질을 인식하는 Goat Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. WB, ICC/IF, IP에 사용 가능하며, 액상 형태로 제공됩니다. TBS 버퍼(pH 7.3, 0.5% BSA) 내 보관하며, 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.01–0.03 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Immunoprecipitation (IP) | 1:10–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide with sequence PFSSDLQKTFEIEL, from C Terminus |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2154284 |
Product Specific Information
- Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX24147-PEP).
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells.
Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex.
NUMB is also expressed in postmitotic neurons and plays a role in axonal growth.
Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca²⁺ channel (VGCC) inhibitor or by Ca²⁺ chelator.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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