Thermo Fisher Scientific PCSK2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

-

Immunocytochemistry (ICC/IF)

1:100-1:400

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

synthetic peptide GLQDSSTDNRAKLC, corresponding to amino acids 95 - 107 of Human PC2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.36 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2134316

Product Specific Information

PA1-31853 has been successfully used in immunofluorescence and Western blot applications, where a band of approximately 82 kDa is detected.

Target Information

The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous approximately 10 kDa amino-terminal proregion, a highly conserved approximately 55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.