Thermo Fisher Scientific Phospho-Elk-1 (Ser383) Polyclonal Antibody
Phospho-Elk-1 (Ser383) 항체로, 인간·마우스·랫트 시료에서 인산화된 Elk-1 단백질 검출에 적합합니다. Western blot 및 IHC에 사용 가능하며, Protein A 정제된 고품질 rabbit polyclonal 항체입니다. -20°C 보관, 반복 동결·해동은 피하십시오.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence including phosphorylation site Ser383 (T-L-S(p)-P-I) derived from human Elk-1, conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | Dulbecco’s PBS (pH 7.4) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of serine/threonine motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 is a direct target of activated MAP kinase. Biochemical studies show ELK1 is a good substrate for MAP kinase, with phosphorylation kinetics correlating with MAP kinase activity. Interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 functions as a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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